U.S. researchers studying zebrafish have found that riboflavin, also known as vitamin B2, may mitigate the toxic effects of cyanide.
Zebrafish. The inset shows riboflavin powder (Fraser B et al / Patrícia R / CC BY-SA 3
The study, reported in the FASEB Journal, also shows that zebrafish are a viable model for investigating the effects of cyanide on humans.
“As with any research involving animal models, these findings are preliminary until thoroughly tested in clinical trials. Anyone who suspects cyanide poisoning should not attempt to use riboflavin as an antidote, and instead contact local poison control centers or emergency health services immediately.”
“We are encouraged to see that many of the effects of cyanide on zebrafish mirror the effects on humans. Hopefully, the cyanide biomarkers and antidotes we discover with the help of zebrafish can one day improve our ability to diagnose and treat humans affected by cyanide poisoning,” said lead author Dr Randall Peterson from the Harvard Medical School in Boston.
To make the discovery, the team exposed zebrafish to cyanide and measured the effects on their behavior, heart rate and survival. The chemical changes that occurred were measured using a mass spectrometer.
The effects in zebrafish were then compared to the effects of cyanide on rabbits and humans. Many of the effects in zebrafish matched those seen in rabbits and humans, confirming that the zebrafish could be used as a model of human cyanide exposure.
From there, the researchers systematically tested thousands of known drugs to see if any of them could protect the zebrafish from cyanide toxicity and found four drugs, including riboflavin.
The study also identified new biomarkers that indicate cyanide exposure and may be clinically useful for the diagnosis of cyanide exposure and the development of antidotes.
“Lottery winners are not the only ones who have to worry about cyanide poisoning. Cyanide exposure also occurs through smoke inhalation, industrial accidents, acts of war, and even as an unwanted byproduct of useful drugs. Therefore, the development of antidotes is crucial, and it’s nice to know that a likely candidate is already on the drugstore shelf,” said Dr Gerald Weissmann, Editor-in-Chief of the FASEB Journal.
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Bibliographic information: Anjali K. Nath et al. Chemical and metabolomic screens identify novel biomarkers and antidotes for cyanide exposure. FASEB. J. 27: 1928-1938; doi: 10.1096/fj.12-225037
